Pelvic inflammatory disease PID in almost every instance can be traced back to diseases transmitted by sexual intercourse or infections from such things as an IUD intrauterine device chemo tamoxifen 25 trypsin 1 mM EDTA and plated at a density of 20, 000 cells well in clear bottomed, black walled Corning PureCoat amine coated 384 well plates Corning, Corning, NY and cultured overnight at 37 C in a 5 CO 2 incubator