To establish LTLT Ca overexpressing EBP1 stable transfectants, subconfluent cells in 100 mm tissue culture dishes were transfected with 10 ?? g of pAGFP1 Hyg C1 or pAGFP1 Hyg C1 EBP1 or pcDNA GFP Hyg or pcDNA GFP Hyg EBP1 T261A expression plasmids using Fugene 6 Roche, Indianapolis, IN, USA according to the manufacturer s protocol clomiphene bodybuilding A, B, C Co transfection of neurosphere derived stem progenitor cells with RFP and empty vector or Mash1 plasmids in vitro







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